Influenza viruses infect our cells, but cell entry alone does not result in influenza (the disease).

What happens after our cells get infected can depend on quite a few things. These include…

1. our innate immune response. It recognises various generalized parts of the virus without having to have a specific memory of the viral strain from a previous infection. It can then respond with a range of antiviral measures
2. whether we have a pre-existing immune memory of the virus. These antibodies and cells can awake to fend off a foothold situation
3. our age. Children have had fewer infections than adults so children succumb more often/easily
4. the strain of Flu virus and whether it is more able to dodge, or even over-stimulate, our immune response; where in our body it prefers to replicate; how good it is at replicating
5. which past Flu epidemics and pandemics we may have survived (related to No. 2, 3 and 4) because this affects how susceptible we are to the latest subtypes or strains; it leaves an imprint
6. how much virus lands on how many cells (includes how many exposures to the virus and how long each exposure is)
7. how the virus arrives; via direct contact, droplet or aerosol (also called airborne or droplet nucleus transmission), self inoculation or a mix
8. where the virus is introduced. Virus may enter via the cell eyes, throat, nose, lungs etc.

Quick facts about Flu virus incubation 

We incubate Flu virus for about 2 days after infection.

Virus usually reaches peak levels within 3 days after infection, reaching slightly higher levels in children than adults, and stopping after about a week.[1]

Shedding of Flu virus can last as long as 3 weeks, but more commonly stops within a week after signs and symptoms start.[1,23] Shedding can last longer in very sick infants.[1]

The next person we infect during influenza virus transmission chain can begin to show symptoms at around 2 to 4 days. For the SARS coronavirus, this range was 8 to 10 days.[1]

So we quickly reach peak virus levels and become infectious. These numbers all come as part of a range – higher or lower, longer or shorter all being possible. In those with mild or no symptoms, peak Flu virus levels are generally lower than in those who have clear signs and symptoms.[1]

Influenza virus transmission: from me to you

Flu viruses reportedly transmit via propelled droplets (≥5μm), aerosols [7] or after contact with infected people or contaminated surfaces and objects onto which virus-laden droplets have settled.[1]

It may amaze some to learn that 1 of every 3 adults have been observed to nose-pick or eye-rub each hour.[21] And not just once per hour. One study found that people touched surfaces and their mouth/nose 3 to 4 times per hour![24] That’s how those surfaces are thought to come into play; our fingertips become contaminated and then we inoculate ourselves with Flu virus.

Hand washing is thought to have some importance (perhaps nothing like as much as aerosols) but that’s a lot of touches to interrupt. Holding off on touching our faces until after we can wash our hands is a good habit to develop.

As well as the virus itself, environmental conditions impact how long Flu virus can remain infectious on surfaces and in these droplets and droplet nuclei.[12,13] Flu viruses can also remain infectious on nonporous surfaces for some time in general. We come into contact with these kinds of materials many times each day.[2,3] Unless we live on an island near a Jedi temple.

Infection without illness

Occasionally influenza virus transmission results in infection without us showing any signs or feeling any symptoms of having become a virus taxi.

Among these outcomes – called subclinical or asymptomatic – if we looked hard enough we might sometimes find a small temperature rise or a bit of a sniffle or tickly throat that we shuck off as nothing. But we seldom think about that. We can also get mild common cold-like illness from a Flu virus infection. We certainly wouldn’t go to a Doctor for these but we would go to school, work, parties and shopping.

Mild or asymptomatic outcomes from Flu infection can occur in as many as 50-77% of infected people.[1,22]

The ferret nose

Studies using ferrets – a small animal model that can be infected using human Flu viruses and show relevant illness – identified that the amount of virus in the nose correlated with influenza virus transmission from infected to uninfected ferrets.[20] Obvious signs of ferret sickness were not necessary. Droplets from symptomatic ferrets late in the disease course did not result in infection of sentinel uninfected ferrets but direct contact usually did (as it did in the early stages too).

Virus can get out before you suspect it

Flu viruses may also transmit during the “presymptomatic” period. This is the time between when we’ve been infected and virus is replicating in our airways but we’re not yet showing signs of the illness to come. Presymptomatic ferrets could produce infectious droplets (and aerosols?).[20] And direct contact (which is pretty direct among ferrets) was a transmission risk before and during symptoms. Another reminder to wash those hands (or paws, if you’re a ferret)!

One study found that neither contact tracing nor isolation of infected people would likely prevent an epidemic. Asymptomatic/presymptomatic spread of infection is a good reason for this.[4]

But hang on a tick. How can Flu virus be transmitted from a person or an animal if they are infected but not coughing or sneezing out droplets and aerosols and contaminating surfaces?

No coughing or sneezing but we all do some breathing

Good question. One way might be through breathing and talking – yes, it might be that easy.[5,6,8,9]

Studies have used different methods to collect breath from virus-infected humans and found virus genetic material. Commonly this is RNA from viruses that have RNA genomes like Flu viruses, respiratory syncytial viruses, rhinoviruses, coronaviruses and parainfluenzaviruses.[7,8,18]

Viruses were not able to be grown in culture, or culture was not used in these studies and this is a limitation because we can’t say with certainty that viruses were breathed out during such studies could infect a susceptible person; we don’t know if the positive results mean infectious virus was present.

ASIDE: PCR methods are used to detect viral RNA. These may be too sensitive to define the period during which a person is infectious, as was found in a study that used ferrets.[10]

Flu virus RNA, parainfluenza virus-3 RNA or rhinovirus RNA has been detected from infected ill subjects breathing only through their noses or their mouths, and/or while talking or coughing.[8,9]  Rhinovirus RNA was also collected from the breath of talking or breathing infected but asymptomatic subjects.[9]

In a study of 2 ill subjects, infectious rhinovirus was produced as shown by virus replicating in laboratory cell cultures from samples of exhaled nasal breath and coughing breath.[8] Flu isn’t the only respiratory virus that may spread via hot air.

So how big is the risk of transmitting virus by breathing?

Despite Flu RNA being detected in 81% of infected people with coughs in a different study, infectious Flu virus was only recovered from 2 of 21 people’s cough aerosols.[11] Another study found RNA in a lot, but recovered infectious Flu virus from only 2 naturally infected volunteers; those with the highest levels of Flu virus RNA in their breath.[5] Intriguingly, the authors also reported more Flu virus RNA in the smaller droplet nuclei than in larger droplets.[5,13]

These findings confirm that infectious virus can be produced from breathing alone, but that only a fraction of those infected seem to do this.

But let’s not sideline this route too much. The most infectious 20% of children and adults in one study, were responsible for 80% of infectiousness (infectious = amount of Flu virus RNA being shed).[23] Even when only a few infected asymptomatic people shed infectious Flu virus, each of those few may infect several or more others.

ASIDE: All of this work is heavily impacted upon by biological variability. These results apply using these particular methods, those scientists, that subject group, their experimental conditions and those Flu virus strains. Interesting hybrid detection methods can help address the RNA vs. virus detection issue, improving the sensitivity of detecting infectious virus.[17] Such techniques can be useful to smooth out some of the differences between studies and to improve upon past studies that only detected RNA.[17]

The range of an aerosol

A final topic on Flu virus transmission; distance. Flu virus RNA has been detected in air collected 1.8m (6 feet) away from ill patients.[18] The authors proposed that there may have been enough infectious virus in that air to cause an infection.

Research from 2014 showed bacteria-laden cough aerosols could travel 4m (13 feet!) and retain bacterial infectivity.[19] Also, aerosols can hang around (literally) until they impact something. I’d wager that viruses could manage a similar feat.

Management of humidity, filtration, flow rate and pressure differences of room air can provide important protections against the spread of infection within healthcare settings. Appropriate personal protective equipment is also important to reduce exhaled or expelled infectious particles and protect us from those expelled by other infected people.[5] This is known.

Droplets and aerosols add to what is clearly a complex Flu equation.

So What? Being infected but looking healthy is nothing to sneeze at

Aerosols play an important part in the transmission of Flu viruses. Virus can be recovered from asymptomatic folks and breathing and talking are the likely ways transmission occurs before anyone around us knows we are sick.

Given that we keep on shedding virus for days after we’re infected, it’s no surprise that office-workers who take just 1 or 2 days off may feel well enough to once again face the workload, but they’re probably infecting their co-workers and helping to keep the Flu season alive.

Flu viruses and the Flu thank you for your tireless efforts.

They thank you less if you got a vaccine which may have reduced the likelihood that a virus gains a foothold in your system or if it does, perhaps reduced the amount of virus you shed, the length of time you shed it or how sick you get at all.

References…

1. Nonpharmaceutical Interventions for Pandemic Influenza, International Measures
   https://wwwnc.cdc.gov/eid/article/12/1/05-1370_article
2. Survival of Influenza Viruses on Environmental Surfaces
   https://www.ncbi.nlm.nih.gov/pubmed/6282993
3. Influenza Virus Contamination of Common Household Surfaces during the 2009 Influenza A (H1N1) Pandemic in Bangkok, Thailand: Implications for Contact Transmission
   https://www.ncbi.nlm.nih.gov/pubmed/20879867
4. Factors that make an infectious disease outbreak controllable
   http://www.pnas.org/content/101/16/6146.full
5. Influenza Virus Aerosols in Human Exhaled Breath: Particle Size, Culturability, and Effect of Surgical Masks
   http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003205
6. Influenza Virus in Human Exhaled Breath: An Observational Study
   http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0002691
7. Aerosol transmission of influenza A virus: a review of new studies
   http://rsif.royalsocietypublishing.org/content/6/Suppl_6/S783.long
8. A New Method for Sampling and Detection of Exhaled Respiratory Virus Aerosols
   https://academic.oup.com/cid/article/46/1/93/338992
9. Exhalation of respiratory viruses by breathing, coughing, and talking
   https://www.ncbi.nlm.nih.gov/pubmed/19626609
10. Correlation Between the Interval of Influenza Virus Infectivity and Results of Diagnostic Assays in a Ferret Model
    https://academic.oup.com/jid/article/213/3/407/2459199
11. Measurements of Airborne Influenza Virus in Aerosol Particles from Human Coughs
    http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0015100
12. Influenza Virus Aerosols in the Air and Their Infectiousness
    https://www.ncbi.nlm.nih.gov/pubmed/25197278
13. Ebola virus may be spread by droplets, but not by an airborne route: what that means
    https://virologydownunder.blogspot.com.au/2014/08/ebola-virus-may-be-spread-by-droplets.html
14. Comparison of the Levels of Infectious Virus in Respirable Aerosols Exhaled by Ferrets Infected with Influenza Viruses Exhibiting Diverse Transmissibility Phenotypes
    http://jvi.asm.org/content/87/14/7864.full
15. Influenza Virus Respiratory Infection and Transmission Following Ocular Inoculation in Ferrets
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291616/
16. Ocular Tropism of Influenza A Viruses: Identification of H7 Subtype-Specific Host Responses in Human Respiratory and Ocular Cells
    http://jvi.asm.org/content/85/19/10117.full
17. Enhanced detection of infectious airborne influenza virus
    https://www.ncbi.nlm.nih.gov/pubmed/21663766
18. Exposure to Influenza Virus Aerosols During Routine Patient Care
    https://www.ncbi.nlm.nih.gov/pubmed/23372182
19. Viability of Pseudomonas aeruginosa in cough aerosols generated by persons with cystic fibrosis
    http://thorax.bmj.com/content/69/8/740
20. Transmission of a 2009 H1N1 Pandemic Influenza Virus Occurs before Fever Is Detected, in the Ferret Model
    http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0043303
21. Transmission of rhinovirus colds by self-inoculation
    http://www.nejm.org/doi/full/10.1056/NEJM197306282882601
22. Comparative community burden and severity of seasonal and pandemic infl uenza: results of the Flu Watch cohort study
    http://www.thelancet.com/journals/lanres/article/PIIS2213-2600(14)70034-7/fulltext
23. Heterogeneity in Viral Shedding Among Individuals With Medically Attended Influenza A Virus Infection
    https://academic.oup.com/jid/article/207/8/1281/891314
24. Facing Ubiquitous Viruses: When Hand Washing Is Not Enough
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552529/pdf/cis961.pdf

Views: 32655